BICTEL/e - ULg
Serveur institutionnel des thèses de doctorat

• bactérie
• bacteria
• proteomics
• froid
• cold
• Protéomique

In order to study bacterial adaptation to low temperature, we selected Pseudoalteromonas haloplanktis TAC125, a strain displaying a fast growth rate at temperatures close to 0°C. Its cellular proteins have been compared by 2D-DIGE electrophoresis (fluorescence difference gel electrophoresis) in order to identify proteins that are either over-expressed or repressed under cold stress conditions. These proteins have been identified by mass spectrometry of their trypsic peptides and amino acid sequencing of the fragmented ions (MALDI TOF/TOF MS or nano LC ESI-MS/MS). Using these data, a metabolic pattern of resistance to low temperature has been drawn. The gene for the main protein involved in this adaptation has been cloned and over-expressed in E. coli. This protein, the Trigger Factor, is being characterized with respect to its chaperone function and specific properties.

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